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portrait de Willy BienvenutWilly Vincent BIENVENUT

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Plateforme d'Analyse Protéomique de Paris Sud-Ouest (PAPPSO)
Génétique Quantitative et Évolution - Le Moulon
INRA - Université Paris-Sud - CNRS - AgroParisTech
Ferme du Moulon
F-91190 Gif-sur-Yvette
e-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Phone: +33 1 69 33 23 68
ORCID

 

Positions and education

  • Since 05-2019: CNRS Scientist at PAPPSO/BASE, UMR GQE-Le Moulon, Gif-sur-Yvette, France
  • 2012-2019: Scientist at CNRS, I2BC, Gif-sur-Yvette, France
  • 2010-2012: Researcher in Biology and Mass Spectrometry Application Specialist at CNRS (Fédération de recherche 3115, Gif-sur-Yvette, France)
  • 2006-2010: Mass spectrometry & Proteomics Core Facility Manager at the “Beatson Institute for Cancer Research”, Glasgow, Scotland, UK
  • 2002-2006: Mass Spectrometry application specialist at the “Protein Analysis Facility”, Lausanne University, Switzerland.
  • 2002: Post-doctoral position at the BPRG, Geneva University, Switzerland
  • 1998-2002: PhD Student at the Geneva University hospital, Switzerland
  • 1996-97: Master degree in Material Science at l'INP-ENSC Toulouse, France
  • 1993-96: Chemist engineering degree at INP-ENSC Toulouse, France
  • 1990-92: DUT in chemistry at Poitiers, France.

Main research projects

My research projects are mostly related to proteomics and bio-informatics. My previous development provides an interesting approach for large scale characterisation of protein N-term modification (Bienvenut et al., BMC Bioinformatics, 2017) and N-term acetylation quantitation (Bienvenut et al.,Meth Mol Biol, 2017). This work was at the origin of the N-terDB which provides experimentally characterised N-termini and N-term acetylation yield. Collected data were valorised through the development of new predictions tools that outperform the traditional TargetP or ChloroP (soon available at N-terPred).

I am now working at the Génétique Quantitative et Évolution - Le Moulon. My new interest is related to the determination of large-scale protein turnover in plants using 15N metabolic labelling to better understand plant stress resistance and the pathway involved in draw stress resistance.

Database and prediction tools

  • N-TerDB: experimentally characterised protein N-terminal database for few different species including H. sapiens, A. thaliana, E. coli...
  • N-terPred: Prediction tool for the protein N-terminal maturation including iMet excision, N-term acetylation, transit peptide cleavage site, sub cellular localisation (chloroplast and mitochondria)...

Grant

2014-2018: Coordinator of the ANR project ANR-13-BSV6-0004-01 entitled “Energy conversion organelles N-terminus proteome: A better understanding of organelle protein maturation.”

Keywords

Proteomics, mass spectrometry, bioinformatics, N-terminal acetylation, prediction tools, chloroplast, transit peptide

 

Selected publications

Bienvenut WV, Scarpelli JP, Dumestier J, Meinnel T, Giglione C. (2017) EnCOUNTer: A processing tool to discover organelle-targeted proteins N-termini and to quantify associated N-alpha-acetylation yield. BMC Bioinformatics, 18(1):182. doi: 10.1186/s12859-017-1595-y

Bienvenut WV, Giglione C, Meinnel T. (2017) SILProNAQ: A convenient approach for proteome-wide analysis of protein N-termini and N-terminal acetylation quantitation. Meth Mol Biol,  1574:17-34. doi: 10.1007/978-1-4939-6850-3_3

Frottin F, Bignon J, Bienvenut WV, Jacquet E, Vaca S, Wdzieczak-Bakala J, Van Dorsselaer A, Cianferani S, Carapito C, Meinnel T, Giglione C. (2016) MetAP1 and MetAP2 drive cell selectivity for a potent anti-cancer agent in synergy, by controlling glutathione redox state. Oncotarget,  7(39):63306-63323. doi: 10.18632/oncotarget.11216

Linster E, Stephan IS, Bienvenut WV, Maple-Grødem J, Myklebust LM, Huber M, Reichelt M, Sticht C, Geir Møller S, Meinnel T, Arnesen T, Giglione C, Hell R, Wirtz M. (2015) Proteome imprinting by N-terminal acetylation is a vital hormone-regulated master switch during stress. Nat Commun, 6:7640. doi: 10.1038/ncomms8640

Bienvenut WV, Giglione C, Meinnel T. (2015) Proteome-wide analysis of the amino terminal status of Escherichia coli proteins at the steady-state and upon deformylation inhibition. Proteomics, doi: 10.1002/pmic.201500027

Xu F, Huang Y, Li L, Gannon P, Kapos P, Linster E, Bienvenut W, Polevoda B, Wirtz M, Meinnel T, Hell R, Giglione C, Zhang Y, Chen S, Li X. (2015) Two N-terminal acetyltransferases antagonistically regulate the stability of a nod-like receptor in Arabidopsis. Plant Cell, 27(5):1547-62

Bienvenut WV, Dinh TV, Linster E, Feldman-Salit A, Jung VA, MeinnelT, Hell R, Giglione C, Wirtz M. (2015) Molecular identification and functional characterization of the first Nα-acetyltransferase in plastids by global acetylome profiling. Proteomics, doi: 10.1002/pmic.201500025

Bienvenut WV, Sumpton D., Lilla S., Meinnel T., Giglione C. (2013) Influence of various endogenous and artifactual modifications on large scale proteomics analysis. Rap Commun Mass Spectrom, 27(3):443-50

Bienvenut WV, Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C. (2012) Comparative large-scale characterization of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha-acetylation features. Mol Cell Proteomics,  11(6):M111.015131

Bienvenut WV, Espagne C., Martinez A., Majeran W., Valot B., Zivy M, Vallon O., Adam Z., Meinnel T., Giglione C. (2010) Dynamics of post-translational modifications and protein stability in the stroma of Chlamydomonas reinhardtii chloroplasts. Proteomics, 11(9): 1734-50