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portrait d'Elodie MarchadierÉlodie MARCHADIER

______________________

Génétique Quantitative et Évolution - Le Moulon
INRA, Université Paris-Sud, CNRS, AgroParisTech
Ferme du Moulon
F-91190 Gif-sur-Yvette,
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+33 (0)1 69 33 23 68

 

> Academic history

2005-2009: PhD Cellular Biology - University of Paris XI-Orsay, France - ASC-INRA funding - "Functional exploration of a centre of protein-protein interactions in Bacillus subtilis by an integrative approach"

2003-2005: MSc Bioinformatics - University of Rennes I, France

2000-2003: BSc Cellular biology and molecular genetics - University of Rennes I and ASUC-Chartres, France.

 

> Research interest

I joined BASE group in September 2015 and started to work on the effect of environnment and genotype on maize phenotypes.

Maize genotypes coming from a divergent selection experiment initiated in the group 20 years ago (C. Dillmann, M. Tenaillon, A. Ressayre) were grown in field conditions and characterized for their growth parameters. This material combined with a fine characterization of the environnment provide interesting data to better understand the effect of environnment factors (potential combined stresses) on maize growth phenotypes.

Water availability is a key environnmental factor impacting maize growth. I’m also currently working on the characterization of plant water relations on contrasted maize lines for their drought tolerance in order to provide additional phenotypes to characterize the genotypes of interest.

Having investigate the complexity of genotype-phenotype relation for growth phenotypes in Arabidopsis, I’m also interested in the exploitation of molecular data that underlie phenotypic changes. In this context, I am interested in methods allowing network construction from omics data.

Some biological mecanisms are so extensively studied in different contexts (genotypes, conditions…) that it becomes challenging to integrate the set of regulatory informations which have been described. In this context, I’m interested in text mining approaches enabling the extraction of biological informations for scientific papers (collaboration with Bibliome group) and also in the developement of representation tools of complex biological systems at different scales (molecular, cellular and organism) via the buiding of a systemic plant ontology (collaboration with BioSys group).

 

> Past work

♦ VAST lab, IJBP Versailles: March 2012 to January 2015

My work focused on the genetic variants responsible for abiotic stress adaptation (especially drought stress) in Arabidopsis ecotypes (natural accessions). To this end, we used Recombinant Inbred Lines combined to a high-throughput phenotyping platform, The Phenoscope. This makes possible the accurate quantification of the rosette area and the QTL mappping in finely control growth conditions.

♦ Guard Cell group, University of Bristol: September 2009 to February 2012

Stomata are pores found on leaf epidermis of plants and allow to regulate gas exchange and water loss. Stomata are able to open and close in response to different stimuli such as carbon dioxyde, humidity, light and temperature. The fine tuned guard cell signalling allows the plant to respond to a combination of environmental factors in an optimal manner. I have been focused on how the guard cells can take into account various stimuli to develop an appropriate response.

I have been interested in signals integration which occurs in guard cells and controls stomatal aperture. I studied mechanisms of signal integration which have already been characterized in bacteria and which are conserved in plants. I combined predictive approaches (in silico) such as expression and interaction data to identify potential key proteins and target experimental assays.

In collaboration with Computational Genomics group , I have also been interested in detecting and understanding major steps of signalling pathways evolution using phylogenetic and bioinformatic methods to study how evolution shaped and optimized the biological pathways.

PhD, Institut Micalis and MaIAGE

My PhD focused on an biological question: understanding the biological function of a group of hubs discovered in Bacillus subtilis. These hubs are located at the interface of several essential cellular processes such as  DNA replication, cell division, chromosome segregation, stress response and biogenesis of the bacterial cell wall. I used microbiology approaches combined to bioinformatics and transcriptome analyzes to better characterize these proteins. The integration of these heterogeneous data allowed to clarify the functional context of the genes of interest and to make assumptions about the nature of interactions between hub proteins. The group of hubs appears finally composed of a few groups of co-expressed proteins (party hubs) which can interact together and other proteins expressed in an uncorrelated manner (date hubs).

 

> Teaching activities

I am involved in the teaching of biostatistics and biomathematics at licence and master levels of the Université Paris-Sud.

 

> Publications

- Edel K. H., Marchadier E., Brownlee C., Kudla J., Hetherington A. M. (2017) The Evolution of Calcium-Based Signalling in Plants. Curr Biol, 27 (13) R667-R679. Review.

- Marchadier E, Oates ME, Fang H, Donoghue PC, Hetherington AM, Gough J. (2016) Evolution of the Calcium-Based Intracellular Signaling System. Genome Biol Evol, 8 (7) 2118-32.

- Marchadier E, Hetherington AM.(2014) Involvement of two-component signaling systems in the regulation of stomatal aperture by light in Arabidopsis thaliana. New Phytologist, 203 (2) 462-8

- Monteferrante CG, Mackichan C, Marchadier E, Prejean MV, Carballido-López R, van Dijl JM.(2013) Mapping the twin-arginine protein translocation network of Bacillus subtilis. Proteomics, 13 (5) 800-11

- Nicolas P, Mäder U, Dervyn E, Rochat T, Leduc A, Pigeonneau N, Bidnenko E, Marchadier E, Hoebeke M, Aymerich S, Becher D, Bisicchia P, Botella E, Delumeau O, Doherty G, Denham EL, Fogg MJ, Fromion V, Goelzer A, Hansen A, Härtig E, Harwood CR, Homuth G, Jarmer H, Jules M, Klipp E, Le Chat L, Lecointe F, Lewis P, Liebermeister W, March A, Mars RA, Nannapaneni P, Noone D, Pohl S, Rinn B, Rügheimer F, Sappa PK, Samson F, Schaffer M, Schwikowski B, Steil L, Stülke J, Wiegert T, Devine KM, Wilkinson AJ, van Dijl JM, Hecker M, Völker U, Bessières P, Noirot P. (2012) Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis. Science,  335 (6072) 1103-6.

- Marchadier E, Carballido-López R, Brinster S, Fabret C, Mervelet P, Bessières P, Noirot-Gros MF, Fromion V, Noirot P. (2011) An expanded protein-protein interaction network in Bacillus subtilis reveals a group of hubs: Exploration by an integrative approach. Proteomics, 11 (15) 2981-91.

> International conferences

- Society of Experimental Biology SEB annual meeting « The evolution of calcium signaling » July 2013, Valencia (Spain)

- TranSys ITN Conference « Membrane protein structure, biogenesis and bioinformatics » (Invited speaker) 25-29th October 2010, Maratea (Italy)

- European BaCell Meeting - March 2008, Oslo (Norway)