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portrait de Xacier RaffouxXavier RAFFOUX


Génétique Quantitative et Évolution - Le Moulon
INRA - Université Paris-Sud - CNRS - AgroParisTech
Ferme du Moulon
F-91190 Gif-sur-Yvette
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phone: + 33 (0)1 69 33 68 05


> Education and Positions

Laboratory technician, GQE-Le Moulon (50% ACEP, 50% RAMDAM)
PhD student  GQE-Le Moulon (Gif-sur-Yvette, France), 2013-2018
Master degree in Genetic « Génomes, Cellules, Développement, Evolution», Université Paris-Sud (Orsay, France), 2013
Laboratory technician, GQE-Le Moulon (Gif-sur-Yvette, France), 2003-present
Laboratory technician, LBMC Cellular and Molecular Biology laboratory, (Lyon, France), 2002-2003
Bachelor degree in biology at Université Paris VI (today UPMC), (Paris, France), 1999-2000


> Research Interest

Diversity and genetic determinism of meiotic recombination rate in S. cerevisiae

pictures from experiments

Meiotic recombination is a major driver of genome dynamics and thus evolution in sexually reproducing organisms.
The numbers of crossing-over events and their positions along the chromosomes are tightly regulated, but the mechanisms involved are still not well understood. Getting more insights into the regulation of recombination rate and crossover distribution would be beneficial for many fields of fundamental and applied genetics, in particular to improve the efficiency of plant breeding.

To study the natural genetic diversity of recombination rate, we have set up a high throughput method to measure crossover rates in S. cerevisiae. We constructed 8 tester strains carrying three different fluorescent markers at chosen loci, and we used flow cytometry to measure the segregation of the three markers in the meiospores of hybrids between the testers and yeast strains to phenotype for recombination. Using this method, we extracted the recombination rates and the strengths of crossover interference for a collection of yeast strains representing a large part of the diversity of the species. We also investigated the correlation between recombination rate and sequence similarity between homologs among hybrids.

The genetic determinism of recombination rate will be studied setting up a recurrent selection experiment using high, low or neutral selection pressure on recombination rate. Response to selection will be analyzed and QTL responsible for recombination rate variation detected.

Pictures of experiments

> Publications

Raffoux X., Bourge M., Dumas F., Martin O. C. and Falque M. (2018) Role of cis, trans, and Inbreeding Effects on Meiotic Recombination in Saccharomyces cerevisiae. Genetics, Epub ahead of print.

Raffoux X., Bourge M., Dumas F., Martin O. C., Falque M. (2018) High-throughput measurement of recombination rates and genetic interference in Saccharomyces cerevisiae. Yeast, 35 (6) 431-442.

Boisnard S., Zhou Li Y., Arnaise S., Sequeira G., Raffoux X., Enache-Angoulvant A., Bolotin-Fukuhara M., Fairhead C. (2015) Efficient Mating-Type Switching in Candida glabrata Induces Cell Death. PLoS One, 10 (10) e0140990.

Durand E., Tenaillon M. I., Raffoux X., Thepot S., Falque M., Jamin P., Bourgais A., Ressayre A., Dillmann C. (2015) Dearth of polymorphism associated with a sustained response to selection for flowering time in maize. BMC Evol Biol, 15 103.

Edelist C., Raffoux X., Falque M., Dillmann C., Sicard D., Rieseberg L. H., Karrenberg S. (2009) Differential expression of candidate salt-tolerance genes in the halophyte Helianthus paradoxus and its glycophyte progenitors H. annuus and H. petiolaris (Asteraceae). Am J Bot, 96 (10) 1830-8.

Falque M., Décousset L., Dervins D., Jacob AM., Joets J., Martinant JP., Raffoux X., Ribière N., Ridel C., Samson D., Charcosset A., Murigneux A. (2005) Linkage mapping of 1454 new maize candidate gene Loci. Genetics 170 (4) 1957-66.